Figure 2.
C7 TCR tg CD4+ T cells respond to M. tuberculosis infection in vivo. (A) 104 C7 TCR tg.RAG−/− CD4+ T cells (CD90.1) were transferred into uninfected congenically marked B6 recipients (CD90.2), and splenocytes were stained for CD4 and CD90.1 expression 7 d later. The gate marks donor-derived C7 TCR tg.RAG−/− CD4+ T cells (CD90.1+) cells, and the percentages of these cells within total cells (top) or among CD4+ T cells (bottom) are shown. (B–E) As in A, except mice were aerosol infected 1 d after T cell transfer with 100 CFU M. tuberculosis. pLNs, spleen, and lung were examined 12, 15, and 18 d later. (C) Graphic representation of results from three to four mice per time point. Error bars represent SDs. (D) C7 TCR tg.RAG−/− CD4+ T cells from the indicated organs were harvested from day 15–infected mice, and their ability to make IFN-γ and TNF-α was determined after stimulation with ESAT-6 peptide or medium alone. (E) Intracellular T-bet (shaded histogram) or isotype control staining (continuous line) of C7 TCR tg.RAG−/− CD4+ T cells taken from the indicated organs 15 d after infection. The data presented in this figure are representative of two experiments with three to four mice per group.