Figure 6.
Partition of mRNA in and out stress granules. (A) FISH analysis of mRNA in and out stress granules. HeLa cells were transfected with MS2-tagged β-Gal reporter along with MLN51-CFP as a stress-granule marker. In the bottom panel, cells were treated with arsenite for 30 min. After fixation, cells were hybridized with a MS2 antisense oligonucleotide conjugated to Cy3. Images were captured by confocal microscopy. The GW body (top panel) and stress granule (bottom panel) indicated by arrowheads are enlarged below. (B) Effect of actinomycin D during arsenite recovery. HeLa cells were treated with 0.5 mM arsenite for 30 min and further cultivated in fresh culture medium with or without actinomycin D for 1 h. Stress granules and GW bodies were then stained as in Figure 4.