FIG. 3.
Effects of CCR5 and CXCR4 antibodies and inhibitors on virus replication in primary brain cultures. Chimeric NL4-3 viruses containing the aBR-01, aBL-01, dBR-02, or dBR-07 Envs or the 89.6, ADA, or SG3 control viruses were used to infect primary brain cultures in the absence or presence of 10 μg of CCR5 and CXCR4 monoclonal antibodies (2D7 and 12G5, respectively) per ml, 100 nM TAK-770, and/or 1.2 μM AMD3100, as indicated. HIV-1 replication was monitored by quantitating HIV-1 p24 in culture supernatants. The values represent the means of the results of two experiments (mean ± standard deviation, n = 2).