Figure 5.
Androgen Levels In LuCaP Prostate Cancer Xenografts Grown in Castrate and Intact Mice. A) Testosterone and B) DHT levels were measured by mass spectrometry in castration-sensitive (CS) and castration-resistant (CR) variants of the indicated xenografts. Two to five CS and CR tumors of each line were passaged in non-castrate (intact) or castrate male SCID mice respectively, as indicated. The LuCaP 35 CS→CR samples are xenografts initially passaged as castration-sensitive in intact mice, which then responded to castration with castration-resistant growth. Each data point represents the mean value for an individual xenograft harvested from one mouse; samples were subdivided and assayed in duplicate. Androgen levels were also evaluated in normal kidney and muscle tissues simultaneously obtained from each set of castrate and intact animals. Mean androgen levels for each CS and CR xenografts are presented in Supplemental Table 2.