Figure 2. Light-microscopical methods to distinguish between different populations of nuclei in muscle Cross-section of rat soleus muscle.
(A) with nuclei stained with Hoechst dye 33342 (blue), and with antibodies against laminin (red) and dystrophin (green), illustrating how different types of nuclei can be identified. The region shown within the 40 μm × 40 μm frame is shown at larger magnification in B, either with all channels combined (upper left) or with the channels separated. When examining sections, nuclei with their mass centre inside the dystrophin ring were defined as myonuclei (Myo), while those outside the laminin ring belonged to stromal cells (Str). Nuclei between the rings are probably in satellite cells (Sat).