Figure 3.
Decreased iNKT frequency in periphery of young (3−5 week) mice. Spleen and liver cells from wildtype and CD28 KO mice were harvested at the indicated ages (young mice, 3−5 weeks; old mice, 6−12 weeks) and stained with anti-CD3 and CD1tet (spleen) or anti-CD45, anti-CD3 and CD1tet (liver). Data shown represent a mean ± SE for each group (minimum of 9 mice per group). Spleen and liver NKT cell frequencies in young CD28 KO mice are statistically different from those in young wildtype mice using an exact Wilcoxon rank sum test (p < 0.005).