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The surface density of streptavidin as a function of (A) his6-biotin concentration or (B) SA-AF concentration. PEG-modified surfaces were sequentially exposed to (A) 50 mM Ni2+ solution, different concentrations of his6-biotin solution, and finally 1 μg/ml SA-AF solution or (B) 40 mM Ni2+ solution and 1 mg/ml his6-biotin, followed by different concentrations of SA-AF solution. The lines serve as a guide to the eye.
