Table 2. Primers used for sequencing the OPTN gene.
|
Exon |
Forward primer sequence (5'-3') |
Reverse primer sequence (5'-3') |
| 4 |
TGGAGAGAAAGTGGGCAACT |
CACCAGCTACCACCTATGGA |
| 5 |
GGCATCTTTCAATTCAGAGCC |
GACACGTAAGATTCCACTGC |
| 6 |
TCCCAGAGCTCTGCGATTAA |
GCTACACTGGAATTTCCTCA |
| 7 |
TCTGAGCCACCCCGTTTAAA |
GACCTCCGGTGACAAG |
| 8 |
GGAGAATGTTCTGGAAAGCAG |
GGGTGAACTGTATGGTATCT |
| 9 |
CCCCTGATCCTTTATCCCAA |
AATTCAGTGGCTGGACTAC |
| 10 |
TGGTTCAGCCTGTTTTCTCC |
CCCCCCATCTTACAAGTATTTC |
| 11 |
TGGCCAGGTCTAGTGAAGAA |
TTTATCCCCCTCTCTGAGAG |
| 12 |
GAAATGCTAGTAGGTCGTGG |
CCCTGACCATAGGACATTCA |
| 13 |
CCGGCCAGAGCTGATAAT |
AGATCCACTGAGCACTTTCC |
| 14 |
CTAGCAGGATTGTGCATCGT |
GTGGCGCGAACACAGCTATT |
| 15 |
TTTCCCCTACTTCTGTGGAC |
GAGACTGACGGGTGCTATAT |
| 16 | TCATGTCCCACTACGTGTTG | TGTGCCCGGCCTGTTTTCTT |
Primers used in amplification of OPTN exons were also used in sequencing reactions. Primers located in introns were placed far enough away from the exon boundaries to allow visualization of the sequence of the splice sites. Exons 4 through 16 are the exons that contain coding sequence.