FIGURE 7.
Comparative analysis of the detection of endogenous TonEBP versus the TonEBP-GFP construct. Confocal analysis of endogenous TonEBP staining (columns 1 and 3) in IMCD3 cells maintained at isotonic conditions (300 mosmol/kg H2O) (column 1) or after 30 min of acute hypertonic stress (550 mosmol/kg H2O) (column 3) shows a similar pattern compared with that obtained employing the TonEBP-GFP construct (columns 2 and 4). TonEBP (green) is primarily localized in the nucleus (DAPI; blue) following acute osmotic stress.