Figure 1.
Hypoxia-induced VPF/VEGF expression in M21 melanoma cells. (A) M21 melanoma cells were exposed to normoxic (N) or hypoxic (H) conditions for the indicated times. Total RNA was extracted and analyzed using Northern blot for VPF/VEGF (VEGF) and 36B4 (36B4), ribosome-associated protein, as a control. Hybridization signals for each are indicated and ethidium bromide (Eth. Br.) stained gel is shown. (B) Quantification of the fold induction of VPF/VEGF mRNA signal at the indicated times. VPF/VEGF signals were normalized to 36B4 control for each lane and hypoxic conditions were compared with normoxic conditions. (C) Rate of VPF/VEGF secretion from M21 cells under hypoxic conditions. Conditioned media analyzed for VPF/VEGF using enzyme-linked immunosorbent assay was evaluated for increased VPF/VEGF secretion for the indicated time periods.