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Vps10p is properly localized in a vps35–101 strain but is mislocalized to the vacuole in a vps35–108 strain. VPS35 (LSY6–2A/pLS13), vps35Δ (LSY6–2A), vps35–101 (LSY6–2A/pLS12), and vps35–108 (LSY6–2A/pPB7–010.4) strains were fixed, spheroplasted, and costained with antibodies against Vps10p and Vma2p. After subsequent treatment with fluorochrome-conjugated secondary antibodies, the cells were viewed by DIC optics and by epifluorescence through filters specific for FITC and Texas Red.
