Skip to main content
. Author manuscript; available in PMC: 2008 Aug 22.
Published in final edited form as: Biochemistry. 2006 Sep 19;45(37):11087–11095. doi: 10.1021/bi061237w

Figure 2.

Figure 2

UV-visible CD spectra of A. vinelandii IscFdx (FdIV) (black) and A. vinelandii [2Fe–2S]IscU in the absence (blue) and presence of HscA (green), HscB (magenta), HscA/HscB (cyan), HscA/HscB/MgATP (red). The IscFdx sample was 0.10 mM in both protein and [2Fe–2S]2+ clusters (1.0 [2Fe–2S]2+ clusters/monomer) and the [2Fe-2S]IscU samples were 0.30 mM in IscU monomer and 0.18 mM in [2Fe–2S]2+ clusters (1.2 [2Fe–2S]2+ clusters/IscU dimer). [2Fe-2S]IscU samples treated with HscA, HscB, HscA/HscB, and HscA/HscB/MgATP were 0.30 mM HscA and/or HscB and the MgATP-containing sample was 2.0 mM ATP, and 40 mM MgCl2. Δε values are based on the [2Fe–2S]2+ cluster concentration and the pathlength was 0.1 cm.