Figure 4.
Actin cytoskeleton distribution of log-phase wild-type andgcs1Δ cells. Wild-type (A and B) and gcs1Δ (C and D) cells grown at 30°C were fixed, and stained with rhodamine-conjugated phalloidin as described in MATERIALS AND METHODS. Cells were viewed with Nomarski optics (left) and fluorescence rhodamine filter (right) with a 100× oil immersion objective. Representative fields are shown; note the increase in the number of actin patches in gcs1Δ. Focal planes were chosen to maximize the number of actin patches observed.