TABLE 1.
Summary of Proliferation and Differentiation Characteristics of M–/– Macrophages Expressing Y → F Mutant CSF-1Rs
| Cell line | Doubling time (h) | % M–/–.WT expression of Mac-1 |
|---|---|---|
| M–/–.WT | 26.4 ± 1.9 | 100 ± 15 |
| M–/–.vec | NA | 12 ± 4a,b |
| M–/–.YEF | NA | 23 ± 7a,b |
| M–/–.Y544F | 35.1 ± 1.7a | 67 ± 7a |
| M–/–.Y559F | 45.2 ± 2.0a | 31 ± 3a |
| M–/–.Y697F | 28.6 ± 1.4 | 66 ± 14 |
| M–/–.Y706F | 29.8 ± 2.6 | 154 ± 7a |
| M–/–.Y721F | 29.4 ± 1.8 | 67 ± 9 |
| M–/–.Y807F | 78.2 ± 3.3a | 23 ± 13a |
| M–/–.Y921F | 24.0 ± 1.9 | 51 ± 10a |
| M–/–.Y974F | 27.0 ± 2.1 | 67 ± 12 |
Results of multiple growth curves and differentiation assays (n=3) for each cell line.
a
Significantly different from M–/–.WT macrophages (P<0.05; Student’s t-test).
b
Results for residual, viable cells. NA, not applicable, cells fail to survive in CSF-1.