Table 1.
Examples of cryo-EM maps of various specimens studied at different electron accelerating voltages achieved at subnanometer resolutions expressed in terms of a fraction of Nyquist frequency using the Gatan 4k × 4k CCD camera.
| Specimen | Voltage (kV) | Effective Magnification | Å/pixel | Map Resolution (Å) | Resolution as a fraction of Nyquist frequency |
|---|---|---|---|---|---|
| Adenovirus (Saban et al., 2005) | 300 | 253,654 × | 0.59 | 8.9 | 0.13 |
| Adenovirus (Saban et al., 2006) | 300 | ≥253,654 × | 0.59 | 6.9 | 0.17 |
| Cytoplasmic polyhedrosis virus (Booth et al., 2004) | 200 | ~83,100 × | 1.81 | 9.0 | 0.40 |
| Flagellar filament (Yonekura et al., 2006) | 300 | ~110,00 × | 1.35 | 8.0 | 0.34 |
| GroEL (Chiu et al., 2006) | 200 | ~110,800 × | 1.34 | 9.0 | 0.30 |
| GroEL (Stagg et al., 2006) | 120 | 66,400 × | 2.26 | 7.8 | 0.58 |
| Pyruvate dehydrogenase complex (Yu et al., 2008) | 200 | 69,220 × | 2.17 | 8.8 | 0.49 |
| Ryanodine receptor (Ludtke et al., 2005) | 200 | ~83,100 × | 1.81 | 9.6 | 0.38 |