FIGURE 7.
Effect of PKC activator Bryostatin1 on Kv3. 3 mutants S3D, S3C, S3T, and S9D. A, representative current traces of Kv3.3 mutants S3D, S3C, S3T, and S9D before and 20 min after exposure to Bryostatin1. Like S3A, S3D and S3C also produced noninactivating currents, and application of Bryostatin1 caused a small decrease in the peak current amplitudes at the different voltages tested. The response of mutant S3T Kv3.3 channels was similar to that of wild type Kv3.3 channels. B, group data for the effects of Bryostatin1 on peak and inactivated components of current at +40, +50, +60, and +70 mV for mutations at serine 3 (S3D, S3C, and S3T). C, group data for the effects of Bryostatin1 on peak and loss of inactivation of current for the S9D mutation (n = 8 and 5 for wild type and S9D, respectively). ***, p < 0.0005. Protocols for measurements of peak and loss of inactivation of current are as described in the text.