FIGURE 6.
Effect of PKC activator Bryostatin1 on Kv3. 3 mutants. A, representative current traces of Kv3.3 mutants, Δ78, S3A, S4A, S9A, S32A, S33A, S46A, S53A, and S60A, and the effect of Bryostatin1 on peak currents and on inactivation of current after 20 min of drug application. Δ78 and S3A produce noninactivating currents. Some mutations (e.g. S3A) produced currents in which rapid activation was followed by a very rapid (∼20 ms) but only partial decline to a steady state, as has also been described for wild type Kv3.1 currents (31). B, group data for the effects of Bryostatin1 on peak currents of wild type Kv3.3 channels and on Kv3.3 mutants Δ78, S3A, S4A, S9A, S32A, S33A, S46A, S53A, and S60A. For simplicity only currents evoked in response to voltage commands to +70 mV are quantified. C, group data for the effects of Bryostatin1 on the inactivated component of currents at +70 mV for the same mutants as in B. Data were analyzed as described in Fig. 5. For statistical analyzes, the change in peak currents and loss of inactivation of currents for the different mutant Kv3.3 channels were compared with those for the wild type Kv3.3 channels. **, p < 0.01; ***, p < 0.005. Numbers of observations (N) are indicated on the bar graphs.