Table 4.
Effect of palmitate on rates of glucose oxidation and palmitate oxidation by isolated diaphragms from wild-type mice and PDK4−/− mice fed high-fat diet
| Measurementsa | Addition, mmol/l |
Genotype |
|
|---|---|---|---|
| Wild type | PDK4−/− | ||
| Glucose oxidation | None | 2.01±0.17 | 3.02±0.06* |
| Glucose oxidation | Palmitate (0.6) | 1.74±0.15** | 3.06±0.18* |
| Palmitate oxidation | Glucose (5.0) | 0.44±0.03 | 0.33±0.01* |
Data are means ± SE and in μmol·h−1·g wet wt−1; n = 3 in each group.
a
Diaphragms were obtained from overnight-fasted mice. Glucose oxidation was measured by 14CO2 production from [U-14C]glucose, palmitate oxidation by 14CO2, and radioactive acid-soluble product formation from [1-14C]palmitate.
*
P < 0.05 relative to diaphragms from wild-type mice by Student's unpaired t-test;
**
P < 0.05 relative to diaphragms from wild-type mice incubated without palmitate by Student's paired t-test.