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. 2008 Jun 2;28(15):4843–4850. doi: 10.1128/MCB.02214-07

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FIG. 4. — Testing of the conditional Vegfr3^lx allele. (A) The PGKCre; Vegfr3^lx/lz embryo at E10.5 is compared to a control embryo. (B) Western blot analysis of protein extracted from E9.5 PGKCre; Vegfr3^lx/lx and control embryos. The VEGFR-3 protein is not detected in the Cre-positive embryo. (C) Deletion of Vegfr3 in the epiblast produces a null phenotype. The K19Cre; Vegfr3^lx/lz embryo at E10.5 is compared to a littermate control. (D) PCR genotyping analysis of embryos by using primers that detect the floxed (lx) and recombined allele (lx*) is shown. A majority of the K19Cre; Vegfr3^lx/lz embryos showed 100% recombination and displayed a null phenotype, while a minority of the embryos and the few surviving mice showed only partial recombination (band *) and no phenotype. (E) Adjacent sections of the E10 Vegfr3^lx/lz control and the K19Cre; Vegfr3^lx/lz embryos stained for PECAM-1 (upper panels) and VEGFR-3 (lower panels).