Figure 3.
Functional classification of identified IMCD phosphoproteins. Heat maps were constructed from the three major Gene Ontology (GO) categories: cellular component (cyan), biological process (yellow), and molecular function (magenta). The percentage of phosphoproteins associated with each individual GO term has been log2 transformed and then converted into a 16-bit grayscale intensity value (min = 0, max = 255). A direct comparison of whole cell lysate (total) with the membrane-enriched fraction (200k) indicates an increased number of phosphoproteins in the 200k fraction that have GO terms associated with membrane localization/membrane transport functions.
