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BCL9 is required for the transcription of endogenous Wnt target genes. Transcript levels of BCL9, B9L, c-MYC and AXIN2, as measured by RT-qPCR, in (A) SW480 cells or (B) Wnt-induced HEK 293 cells (as in Fig. 3), treated with siRNA to deplete β-catenin, BCL9 or B9L. Statistical significance (*, p < 0.01 and **, p < 0.001) relative to controls (set arbitrarily to 100%) is indicated above bars.
