Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 Oct;120(1):407–415. doi: 10.1128/jb.120.1.407-415.1974

Isolation and Characterization of an Escherichia coli K-12 Mutant with a Temperature-Sensitive RecA− Phenotype

Robert G Lloyd a,1, Brooks Low a, G Nigel Godson a, Edward A Birge a,2
PMCID: PMC245777  PMID: 4607624

Abstract

A mutation that causes a temperature-sensitive RecA− phenotype was identified in a derivative of a PolA− strain that failed to grow at high temperature. The mutant allele (recA200) was shown to be linked to cysC, conferred a sharply temperature-sensitive, ultraviolet-sensitive Rec− phenotype in the range 35 to 42 C, and in crosses failed to show complementation at 42 C with Hfr's that transferred recA−. Double mutants that carried both recA200 and polA were examined for ability to grow and synthesize DNA at restrictive temperatures.

Full text

PDF
407

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brooks K., Clark A. J. Behavior of lambda bacteriophage in a recombination deficienct strain of Escherichia coli. J Virol. 1967 Apr;1(2):283–293. doi: 10.1128/jvi.1.2.283-293.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CLARK A. J., MARGULIES A. D. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1965 Feb;53:451–459. doi: 10.1073/pnas.53.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark A. J. Recombination deficient mutants of E. coli and other bacteria. Annu Rev Genet. 1973;7:67–86. doi: 10.1146/annurev.ge.07.120173.000435. [DOI] [PubMed] [Google Scholar]
  5. Clark A. J. Toward a metabolic interpretation of genetic recombination of E. coli and its phages. Annu Rev Microbiol. 1971;25:437–464. doi: 10.1146/annurev.mi.25.100171.002253. [DOI] [PubMed] [Google Scholar]
  6. De Lucia P., Cairns J. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1164–1166. doi: 10.1038/2241164a0. [DOI] [PubMed] [Google Scholar]
  7. Glickman B. W., van Sluis C. A., Heijneker H. L., Rörsch A. A mutant of Escherichia coli K12 deficient in the 5'-3' exonucleolytic activity of DNA polymerase I. I. General characterization. Mol Gen Genet. 1973 Jul 31;124(1):69–82. doi: 10.1007/BF00267166. [DOI] [PubMed] [Google Scholar]
  8. Gross J. D., Grunstein J., Witkin E. M. Inviability of recA- derivatives of the DNA polymerase mutant of De Lucia and Cairns. J Mol Biol. 1971 Jun 14;58(2):631–634. doi: 10.1016/0022-2836(71)90377-9. [DOI] [PubMed] [Google Scholar]
  9. Gross J., Gross M. Genetic analysis of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1166–1168. doi: 10.1038/2241166a0. [DOI] [PubMed] [Google Scholar]
  10. Heijneker H. L., Ellens D. J., Tjeerde R. H., Glickman B. W., van Dorp B., Pouwels P. H. A mutant of Escherichia coli K12 deficient in the 5'-3' exonucleolytic activity of DNA polymerase I. II. Purification and properties of the mutant enzyme. Mol Gen Genet. 1973 Jul 31;124(1):83–96. doi: 10.1007/BF00267167. [DOI] [PubMed] [Google Scholar]
  11. Howard-Flanders P., Boyce R. P. DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes. Radiat Res. 1966;(Suppl):156+–156+. [PubMed] [Google Scholar]
  12. Karamata D., Gross J. D. Isolation and genetic analysis of temperature-sensitive mutants of B. subtilis defective in DNA synthesis. Mol Gen Genet. 1970;108(3):277–287. doi: 10.1007/BF00283358. [DOI] [PubMed] [Google Scholar]
  13. Kelley W. S., Whitfield H. J. Purification of an altered DNA polymerase from an E. coli strain with a pol mutation. Nature. 1971 Mar 5;230(5288):33–36. doi: 10.1038/230033a0. [DOI] [PubMed] [Google Scholar]
  14. LEDERBERG J., LEDERBERG E. M. Replica plating and indirect selection of bacterial mutants. J Bacteriol. 1952 Mar;63(3):399–406. doi: 10.1128/jb.63.3.399-406.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LOW B., WOOD T. H. A QUICK AND EFFICIENT METHOD FOR INTERRUPTION OF BACTERIAL CONJUGATION. Genet Res. 1965 Jul;6:300–303. doi: 10.1017/s001667230000416x. [DOI] [PubMed] [Google Scholar]
  16. LURIA S. E., BURROUS J. W. Hybridization between Escherichia coli and Shigella. J Bacteriol. 1957 Oct;74(4):461–476. doi: 10.1128/jb.74.4.461-476.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Low B. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968 May;60(1):160–167. doi: 10.1073/pnas.60.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Low B. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12. J Bacteriol. 1973 Feb;113(2):798–812. doi: 10.1128/jb.113.2.798-812.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Low B. Restoration by the rac locus of recombinant forming ability in recB - and recC - merozygotes of Escherichia coli K-12. Mol Gen Genet. 1973 Apr 12;122(2):119–130. doi: 10.1007/BF00435185. [DOI] [PubMed] [Google Scholar]
  20. Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Manly K. F., Signer E. R., Radding C. M. Nonessential functions of bacteriophage lambda. Virology. 1969 Feb;37(2):177–188. doi: 10.1016/0042-6822(69)90197-4. [DOI] [PubMed] [Google Scholar]
  22. Monk M., Kinross J. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I. J Bacteriol. 1972 Mar;109(3):971–978. doi: 10.1128/jb.109.3.971-978.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Monk M., Kinross J., Town C. Deoxyribonucleic acid synthesis in recA and recB derivatives of an Escherichia coli K-12 strain with a temperature-sensitive deoxyribonucleic acid polymerase I. J Bacteriol. 1973 Jun;114(3):1014–1017. doi: 10.1128/jb.114.3.1014-1017.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. RILEY M., PARDEE A. B. Nutritional effects on frequencies of bacterial recombination. J Bacteriol. 1962 Jun;83:1332–1335. doi: 10.1128/jb.83.6.1332-1335.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Storm P. K., Hoekstra W. P., de Haan P. G., Verhoef C. Genetic recombination in Escherichia coli. IV. Isolation and characterization of recombination-deficiency mutants of Escherichia coli K12. Mutat Res. 1971 Sep;13(1):9–17. doi: 10.1016/0027-5107(71)90121-7. [DOI] [PubMed] [Google Scholar]
  26. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Willetts N. S., Clark A. J., Low B. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli. J Bacteriol. 1969 Jan;97(1):244–249. doi: 10.1128/jb.97.1.244-249.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wood T. H. Effects of temperature, agitation, and donor strain on chromosome transfer in Escherichia coli K-12. J Bacteriol. 1968 Dec;96(6):2077–2084. doi: 10.1128/jb.96.6.2077-2084.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES