Figure 4.
Digitized film autoradiograms of an 8% polyacrylamide gel from a typical RT-PCR experiment showing (A) amplified products from 50 ng of reverse transcribed total RNA from control (odd-numbered lanes) and schizophrenic cortex (even-numbered lanes). In every case, primers specifically amplified the 201-bp and 225-bp products γ2S and γ2L. Numbers above each pair are brain numbers for identification purposes. (B) PCR-amplified products for G3PDH from the same RT-reaction confirm first-strand synthesis efficiencies and enable semiquantification of expression levels.