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Dose response for the effect of forskolin and forskolin plus PMA on cAMP production in 4B cells (A) and primary cultures of hypothalamic cells (B). Cells were incubated for 20 min with 1 mm IBMX and increasing concentrations of forskolin in the presence and the absence of 1 μm PMA. In both systems, PMA potentiated the stimulatory effect of high concentrations of forskolin on cAMP production.
