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. 2008 Jun 11;105(24):8339–8344. doi: 10.1073/pnas.0801375105

Fig. 4.

Fig. 4.

iNK T cells provide in vivo and in vitro cognate help for antihapten antibody production. (A) B6 WT mice were immunized with two separate injections of 2.2 μg of NP-KLH (i.p.) and 0.5 μg of αGalCer (s.c.) or one injection of 2.2 μg of NP-KLH mixed with 0.5 μg of αGalCer (i.p. only). (B) Comparable mice were immunized with two separate injections of 0.5 μg of NP-βGalCer (i.p.) and αGalCer (s.c) or one injection of 0.5 μg of NP-βGalCer mixed with αGalCer (i.p. only) (n = 5 per group). (C) Three-day culture of CFSE-labeled 1 × 105 Igha B6 CD1d+/+ splenic B cells, 1 × 105 Ighb B6 CD1d−/− splenic B cells, and 1 × 105 iNK T TcR Tg splenic T cells plus media, 50 ng/ml NP-αGalCer, 50 ng/ml αGalCer, 50 ng/ml βGalCer, or 1 μg/ml LPS. IgMa+ CD1d−/− B cells, IgMb+ CD1d+/+ B cells, and Thy1.2+ T cells were identified by FACS. Proliferation represents percentage of cells that have divided from a pool of triplicate wells. Representative of two experiments is shown.