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. 2008 Jul 10;118(8):2722–2732. doi: 10.1172/JCI33713

Figure 7. Increased Smad3/Smad4-mediated Snail transcription contributes to Smad2 loss–associated Snail overexpression.

Figure 7

(A and B) Comparative PCR from chromatin immunoprecipitation (IP) showed an increase in Smad4 binding to the Snail promoter in Smad2–/– skin compared with WT skin. Residue Smad2 binding in Smad2–/– skin was from nonkeratinocyte population of the whole skin. Smad3 binding to the Snail promoter was not significantly changed in Smad2–/– skin in comparison with WT skin. Smad4 binding to the Snail promoter was significantly increased in Smad2–/– skin. *P < 0.05. (C) Dual knockdown of Smad2 and Smad3 or Smad2 and Smad4 abrogated Smad2 loss–associated Snail overexpression. Smad2 knockdown (48 hours) caused a significant increase in Snail expression. Knockdown of Smad4 alone caused a reduction in Snail expression. Concomitant knockdown of Smad2 and Smad3 or Smad2 and Smad4 reduced Snail expression back to mock-transfection levels. †P < 0.05 compared with mock transfection. ‡P < 0.05 compared with Smad2 siRNA treatment.