FIG. 3.
Normal B-cell development in the absence of GABPβ1L. (A) Early B-cell development in the bone marrow. Bone marrow cells were isolated and stained with anti-B220 and IgM. Each population is presented as a percentage of the total number of bone marrow cells. (B) Peripheral T and B cells. Splenocytes were isolated and stained with anti-CD3 and anti-B220. The percentages of T and B cells in the spleen are shown. (C) Marginal-zone (MZ) and follicular B cells. B220+ splenic B cells were further fractionated based on CD21 and CD23 expression. Each population is shown as a percentage of B220+ cells. (D) B-1 and B-2 B cells in the peritoneal cavity. Peritoneal cells were collected and stained with anti-B220 and anti-CD21. Each population is presented as a percentage of gated lymphocytes. (E) B-1a and B-1b B cells in the peritoneal cavity. Peritoneal cells were stained with anti-IgM and anti-CD5 in addition to anti-B220 and anti-CD21. Each population is presented as a percentage of B220+ CD21low B1 B cells. All data are representative of at least four independent analyses of more than six animals of each genotype.