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Growth progress of Yersinia in a 3D-CoG environment at 37°C. WA(pYVO8) and its plasmidless derivative WA-C were studied in 3D-CoG by time-lapse video microscopy (phase contrast) for 20 h, and growth characteristics were compared. WA-C (A to C) grows in small clusters of only a few cells or singly, while WA(pYVO8) forms chains of rods (which start to convolute) and establishes dp spherical microcolonies within 20 h (D to F). Scale bars, 10 μm.
