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Enzymatic identification of soluble GAG
| Treatment | GAG Fraction remaining
|
|||
|---|---|---|---|---|
| Kidney | Liver | Brain | ||
| −/− | −/− | −/− | +/− | |
| Mock-treated | 1.0 | 1.0 | 1.0 | 1.0 |
| Heparan sulfate lyase I | 0.2 | 0.4–0.7 | — | — |
| Heparan sulfate lyase II | 0.1 | 0.2 | 0.9–1.0 | 0.9–1.0 |
| Chondroitin ABC lyase | 1.0 | 1.0 | 0.2 | 0.2 |
| Chondroitin AC lyase | — | — | 0.1–0.2 | 0.1 |
The numbers represent the fraction of GAG remaining in each extract after digestion with lyase, relative to the amount remaining in the mock-treated extract.
