Table 1.
Photophysical parameters of phosphine probes.
| ε[a] (M−1cm−1) | λabs[a] (nm) |
λem[a,b] (nm) |
ΦF[a,bc] | |
|---|---|---|---|---|
| 1 | 6,600 ± 200 | 505 | 515 | 0.00372 ± 0.00005 |
| 1-oxide | 18,800 ± 300 | 505 | 520 | 0.00521 ± 0.00004 |
| 9 | 44,000 ± 1,000 | 501 | 520 | 0.64 ± 0.02 |
Measured in phosphate-buffered saline (PBS), pH 7.0.
λex = 470 nm.
Relative quantum yields of fluorescence (ΦF) measured using fluorescein as the standard.