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. Author manuscript; available in PMC: 2008 Jul 8.
Published in final edited form as: Angew Chem Int Ed Engl. 2008;47(13):2394–2397. doi: 10.1002/anie.200704847

Figure 4.

Figure 4

Fluorescence microscopy of Ac4ManNAz-treated HeLa cells labeled with phosphine 1. a) Top row: live HeLa cells treated with Ac4ManNAz, 1 (FITC channel), and the live cell Golgi marker BODIPY® TR C5-ceramide (Cy3 channel); bottom row: HeLa cells treated with Ac4ManNAz, 1, then fixed, permeabilized and treated with anti-Golgin 97 mouse mAb and goat anti-mouse IgG-Alexa Fluor 647 (Cy5 channel). b) Live HeLa cells treated with 1 only. All cells were treated while alive with nuclear stain Hoechst 33342 (DAPI channel) and viability stain propidium iodide (Cy3 and Cy5 channels). The lack of nuclear fluorescence in Cy3 and Cy5 channels indicates propidium iodide exclusion from cells. The scale bars represent 10 μm.