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. Author manuscript; available in PMC: 2008 Jul 11.
Published in final edited form as: Cell. 2008 Jan 11;132(1):43–54. doi: 10.1016/j.cell.2007.11.045

Fig. 5. The ssDNA Binding Tyrosines of β a27e Important to Function.

Fig. 5

(A) Replication assays contain the indicated amount of Pol III* and β (or mutant β). The plot shows the quantitation of DNA synthesis using either: βWT (blue diamonds), βY154A (red squares), βY153A (orange triangles), or β Y154A/Y153A (green circles). (B) Polymerase extension rate was determined using primed M13 ssDNA to which β, or mutant β, is assembled on the DNA followed by initiating synchronous chain extension and native agarose gel analysis of products after quenching at the indicated times. (C) Bead based clamp loading rate assays were performed using either βWT (blue), β Y154A (red), β Y153A (orange), or β Y154A,/Y153A (green). For the purpose of comparison, the purple squares are the result using βR24A,Q149A.