Figure 2. Representative data from EMSA of E3Q Fpg binding to OG-, Gh- and Spcontaining 30-bp duplexes illustrating relative affinity for the different lesions and changes with opposite base.

A) Plot of percent E3Q Fpg bound to duplex containing a central OG:C (closed black squares), Gh:C (closed red circles) or Sp:C (closed green triangles) base pairs as a function of E3Q Fpg concentration at 25 °C.
B) Plot of percent E3Q Fpg bound to duplexes containing a central Gh:G (closed red circles) or Sp:G (closed green triangles) DNA as a function of E3Q Fpg concentration at 25 °C.