Table 1.
The Lumbricus rubellus Expressed Sequence Tag dataset.
| Library | Earthworm treatment regime1 | Number of ESTs | Mean EST length (bp) ESTs ± SD | No. of clusters (putative genes)2 | Mean consensus length (bp) | Mean translation length (aa) | Redundancy (ESTs/gene) |
| 1 | Adult worms collected from a control field site and acclimatised to test condition in the laboratory | 1173 | 550.4 (± 132.7) | 718 | 759.9 (± 350.7_ | 195.5 (± 103.5) | 1.634 |
| 2 | Late developmental stage (~5 weeks post laying) embryonic tissue dissected from cocoons laid by paired unexposed adult worms | 2728 | 622.6 (± 85.9) | 1314 | 772.4 (± 291.6) | 148.7 (± 95.4) | 2.076 |
| 3 | ~40 day post hatch juveniles (at mid log growth phase = approximately 300 mg) reared from hatchling emerging from cocoons laid by paired unexposed adult worms | 2859 | 405.8 (± 125.9) | 1757 | 598.0 (± 328.0) | 106.5 (± 84.7) | 1.627 |
| 4 | Head enriched (anterior segments 1–33) from acclimatised adult worms | 2569 | 537.5 (± 173.0) | 1357 | 655.6 (± 301.8) | 114.4 (± 89.6) | 1.893 |
| 5 | Mix of tissue from acclimatised adult worms exposed to either 50, 200 or 600 mg Cd/kg dry soil | 2230 | 405.8 (± 125.9) | 1548 | 710.9 (± 291.6) | 113.7 (± 84.2) | 1.441 |
| 6 | Mix of tissue from acclimatised adult worms exposed to either 62, 140, 316, 711 and 1066 mg FLA/kg dry soil | 2336 | 566.7 (± 133.1) | 1625 | 732.3 (± 307.6) | 145.9 (± 96.8) | 1.438 |
| 7 | Mix of tissue from acclimatised adult worms exposed to either 12 and 35 mg ATZ/kg dry soil | 1739 | 568.2 (± 169.1) | 1386 | 706.0 (± 299.5) | 124.5 (± 91.7) | 1.255 |
| 8 | Mix of tissue from acclimatised adult worms exposed to either 40, 160, 460 and 480 mg Cu/kg dry soil | 1518 | 544.0 (± 176.9) | 1084 | 662.0 (± 283.1) | 122.3 (± 85.7) | 1.400 |
| 9 | Reproductive organs (following a subtractive hybridisation protocol) | 73 | 321.2 (± 94.9) | 66 | 372.7 (± 212.2) | 87.3 (± 58.3) | 1.106 |
| Total | 17225 | 539.7 (± 155.0) | 8129 | 611.0 (± 243.0) | 107.4 (± 74.9) | 2.119 | |
1. See Additional File 1 for more detailed descriptions of the libraries used.
2. A cluster is counted as derived from a library if it contains at least one EST from that library. Thus the longest cluster (LRC00201, encoding paramyosin) contains ESTs from five of the nine libraries and counts as a member of all five.