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. 2008 Jun 3;105(23):8067–8072. doi: 10.1073/pnas.0709684105

Fig. 2.

Fig. 2.

Inhibitors of PLA2 and HDAC prevent IL-1β secretion. (A and B) Monocytes were stimulated with the different PAMPs or DAMPs for 3 h in the absence or presence of the PLA2 inhibitors BEL (10 μM; empty columns) or AACOCF3 (AA, 40 μM; filled columns) (A) or for 15 h in the absence or presence of the HDAC inhibitors ITF2357 (100 nM; empty columns) or trichostatin A (TSA, 1 μM; filled columns) (B). Secreted IL-1β was quantified by ELISA. Data are expressed as percent of secretion with respect to untreated cells. Values are the mean ± SD of at least three experiments on monocytes from different donors (P < 0.001 and P < 0.01 in A and B, respectively). (C) Western blot analysis of supernatants from monocytes cultured for 3 h with LPS plus MDP (lanes 1, 3, and 5) or zymosan (ZYM; lanes 2, 4, and 6) in the absence (lanes 1 and 2) or presence of AACOCF3 (AA; lanes 3 and 4) or BEL (lanes 5 and 6). Migration of the 35-kDa pro-IL-1β and the 17-kDa mature IL-1β molecular forms is indicated.