TABLE 1.
S. pombe strains used in this study
| Strain name | Related genotype | Source or reference |
|---|---|---|
| SP129 | Same as Lindner KYG461: h+his3-D1 leu1-32 ura4-D18 ade6-M210 | ATCC 96116 |
| SP185 | h+rad2::ura4−ade6-704 ura4-D18 | 66 |
| SPN341 | h−Msmt-0 nbs1Δ::kanMX6 leu1-32 ura4-D18 ade6-M216 | This study |
| TY116 | h−Msmt-0 ura4-D18 pat1-114 | This study |
| TY64 | Same as TY116 but nip1Δ::ura4+ | This study |
| TY94 | Same as TY116 but nip1Δ::ura4+rad32Δ::ura4+ | This study |
| TY117 | Same as TY116 but rad32Δ::ura4+ | This study |
| TY144 | Same as TY116 but rec12Δ::kanMX | This study |
| TY145 | Same as TY116 but nip1Δ::ura4+rec12Δ::kanMX | This study |
| YA119 | h−Msmt-0 ura4-D18 leu1-32 his3-D1 arg3-D1 | 1 |
| YA1077 | Same as YA119 but rad32Δ::kanMX6 | This study |
| YA1097 | Same as SP129 but nip1Δ::ura4+ | This study |
| YA1128 | Same as SP129 but nbs1-s10 | This study |
| YA1177 | Same as SP129 but rhp51Δ::kanMX6 | This study |
| YA1184 | Same as SP129 but nbs1Δ::kan | This study |
| YA1233 | Same as SP129 but nip1Δ::ura4+nbs1Δ::kanMX6 | This study |
| YA1268 | Same as SP129 but rhp51Δ::his3+nip1Δ::ura4+ | This study |
| YST046 | Same as YA119 but nip1Δ::ura4+ | This study |
| YST153 | Same as YA119 but nip1+::3×FLAG | This study |
| YST167 | Same as YST153 but rad3Δ::ura4+ | This study |
| YST177 | Same as YST153 but tel1Δ::ura4+ | This study |
| YST194 | Same as YST153 but nbs1Δ::kanMX6 | This study |
| YST200 | Same as YST153 but rad32Δ::kanMX6 | This study |
| YST208 | Same as YST153 but chk1Δ::ura4+ | This study |
| YST212 | Same as YST153 but rad26Δ::ura4+ | This study |
| YST233 | Same as YST153 but rhp51Δ::his3+ | This study |
| YST236 | Same as YST153 but cds1Δ::ura4+ | This study |
| YST237 | Same as YST153 but rad17Δ::ura4+ | This study |
| YST242 | Same as YST153 but rad1Δ::ura4+ | This study |
| YST244 | Same as YST153 but rad9Δ::ura4+ | This study |
| YST254 | Same as YST153 but mrc1Δ::ura4+ | This study |
| YST258 | Same as YST153 but crb2Δ::ura4+ | This study |
| YST266 | Same as YST153 but rad3Δ::ura4+tel1Δ::ura4+ | This study |
| YST271 | Same as YST153 but chk1Δ::ura4+cds1Δ::ura4+ | This study |
| YST438 | Same as YA119 but nip1+::3×FLAG::kanMX6 | This study |
| YST442 (SQTQ) | Same as YA119 but nip1 S35A T175A::3×FLAG::kanMX6 | This study |
| YST445 (SP1SP2) | Same as YA119 but nip1 S114A S165A::3×FLAG::kanMX6 | This study |
| YST447 (SQTQSP1SP2) | Same as YA119 but nip1 S35A S114A S165A T175A::3×FLAG::kanMX6 | This study |