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. 2004 Oct 19;91(9):1711–1717. doi: 10.1038/sj.bjc.6602201

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Copyright © 2004 Cancer Research UK

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Double staining for CD8 (red) and Ki-67 (brown) (A). Green arrows indicate double positive cells (nucleus labelled in brown, cell surface in red). Red arrows indicate CD8+ T cells without labelling for Ki67. Arrowheads indicate single positivity for Ki-67 in cancer cells. Scale bar, 20 μm. Comparison of the labelling index of Ki-67 between intraepithelial and peritumoral CD8⁺ T cells (B).