Figure 2.
luh-1 develops normal flowers but exhibits defects in vegetative growth. A, A wild-type (Columbia erecta-105) flower. B, A luh-1 flower in the Columbia erecta-105 background. C, Germination of wild-type seeds on MS medium at 5 d. D, Germination of luh-1 seeds on MS medium at 5 d. E, Three-week-old wild-type and luh-1 plants. F, Root elongation of wild-type and luh-1 seedlings on MS medium. Germinated seedlings were transferred to MS plates and grown on vertical plates. Photographs were taken after 7 d. G, 35S∷LUH complemented the luh-1 defect in root elongation as shown in transgenic lines 4 and 5. Root length, measured after 7 d, was expressed as mean ± se. H, Germination phenotype of luh-1 is complemented by 35S∷LUH. Germination is expressed as mean ± se. I, Semiquantitative RT-PCR showing the expression of LUH mRNA in the wild type (Columbia erecta-105), luh-1, and two different 35S∷LUH transgenic lines (lines 4 and 5) in the luh-1 background. The ratio between LUH band intensity and that of the ACT2 control band, which is set to 1 in wild type, is shown below each lane.