Figure 3.
Biochemical characterization of the effects of βARK1 gene disruption on extracts from E13 embryos obtained after crossing of heterozygote mice. (a) Northern blot studies on 20 μg of poly(A)+ RNA extracted from whole E13 embryos hybridized with a cDNA probe for βARK1 (Upper) or glyceraldehyde-3-phosphate dehydrogenase (Lower) used as control. (b) Western blot analysis using a rabbit polyclonal antiserum to the COOH terminus of βARK1 (8). Chemiluminescent detection of alkaline phosphatase-conjugated secondary antibody was used. (c) Assessment of GRK activity against rhodopsin (see Materials and Methods).