Fig. 2.
MMP-7 modulation of RTE. A, Estrogen-depleted cells attached on filters were treated with 10 pg/ml MMP-7 (plus APMA) for 15 min in the absence (filled bars) or presence (hatched bars) of excess MMP7Nab-pAb 1 μg/ml added to the luminal solution in a total volume of 0.2 ml 30 min before assays. Levels of RTE were determined using the EVOM epithelial voltohmmeter. B, Modulation of MMP-7 mRNA and MMP-7 protein with antisense oligonucleotides. hEVECs attached on filters were treated for 2 d with 10 μM of the MMP-7 antisense nucleotide (AS) or the RC nucleotide. C, Control (no transfections). Western blots used the MMP7Nab-pAb. C, Estrogen-depleted cells attached on filters were treated for 2 d with the vehicle (C, control, filled bars) or 10 μM MMP-7 ASO nucleotide (hatched bars). Some cultures were also treated with 10 pg/ml MMP-7 (plus APMA) for 15 min before assays. D, Estrogen-depleted (SFM) or estrogen-treated cells (Est) attached on filters were cotreated for 2 d before experiments with the vehicle (C, control, filled bars) or 10 μM MMP-7 ASO nucleotide (hatched bars). In C and D, levels of RTE were determined in the Ussing chamber. Shown are means (± SD) of three to six repeats. a, P < 0.01, compared with SFM control.