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Specificity of CreB-His binding to the cre tag sequence. EMSAs were performed using 1 nM radiolabeled double-stranded cre tag oligonucleotide (oligo) substrate and 1 μg of CreB-His. Cold competition was with double-stranded no cre tag oligonucleotide at 0, 1, or 10 nM (lanes 1 to 3) or cre tag oligonucleotide at 0, 1, or 10 nM (lanes 4 to 6). The free oligonucleotides and shifted forms are labeled. This figure is representative of three experiments.
