FIG. 3.
Effect of anaerobic growth on creD expression. Expression was measured using β-galactosidase reporter plasmid pUB6070 (A) and RT-PCR (B). DH5α(pUB6070) was grown aerobically (AER) or anaerobically (ANAER) to mid-log phase (OD600 = 0.5 to 0.6) in nutrient broth (NB) or in M9 minimal medium containing glucose (GLC) as the sole carbon source. (A) Error bars indicate standard deviations from at least three independent experiments. (B) Bands represent the RT-PCR product separated using agarose gel electrophoresis and visualized under UV transillumination. RT-PCR data are representative of three biological replicates.