FIGURE 7.
Viral RNA inhibition of the endoribonuclease domain of RNase L. (A) Diagrams of RNase L and the constitutively active Δ577 endoribonuclease domain of RNase L. (B,C) FRET reactions containing 100 nM of FRET probe and 300 nM Δ577 RNase L endoribonuclease domain (black line) as described in Materials and Methods. FRET reaction containing 100 nM of probe without RNase L or 2–5A (gray line). FRET reactions containing 100 nM of FRET probe, 300 nM Δ577 RNase L, and the indicated concentrations of ciRNAG5761A (B) or wild-type ciRNA (C). Relative fluorescence units (RFU) plotted versus time of incubation.