Figure 2.—
Transcripts encoding both ferritin subunits are increased in iron-limited cells. The abundance of FER1 and FER2 mRNAs was analyzed by quantitative real-time PCR. Strains 17D− and 2137 were cultured in TAP medium supplemented with the indicated amounts of iron. cDNA corresponding to RNA isolated from Chlamydomonas strains (A) 2137 (top) or (B) 17D− (bottom) was used as a template with primers specific to FER1 or FER2. The fold change in abundance of FER1 (left) or FER2 (right) after normalization to CβLP and relative to the abundance in a 20-μm grown culture was calculated according to the 2−ΔΔCT method (Livak and Schmittgen 2001). Each data point is the average of technical triplicates and represents a separate experiment.