Figure 3.
Induction of iniA after treatment with different antibiotics. Autoradiographs of a Northern blot containing RNA from M. tuberculosis cultures treated either with no antibiotics, 0.01 μg/ml isoniazid, 0.1 μg/ml isoniazid, 1 μg/ml isoniazid, 5 μg/ml ethambutol, 5 μg/ml streptomycin, and 5 μg/ml rifampin. The blots were hybridized first with an iniA DNA probe (Upper) to examine iniA induction; the blot was then stripped and rehybridized with a 16S probe (Lower) to confirm equal RNA loading.