Table 2.
Distribution of rate constants for isotope exchange at peptide amide bonds in rhm-CSFβ (N) and CN157,159-modified rhm-CSFβ (M) (k, min−1)
| Segment | Possible NHs | Very fast k > 10 | Fast 1 > k > 0.1 | Slow 0.1 > k > 0.01 | Very slow k < 0.001 |
| 4–19 (N) | 15 | 10.3 | 0.4 | 0.7 | 3.6 |
| 4–19(M) | 10.3 | 0.6 | 0.7 | 3.4 | |
| 37–55 | 17 | 5.8 | 2.4 | 3.2 | 5.6 |
| 5.8 | 3.3 | 2.6 | 5.3 | ||
| 56–62 | 6 | 0.7 | 1.3 | 1.2 | 2.8 |
| 0.4 | 1.5 | 1.2 | 2.9 | ||
| 63–76 | 12 | 5.2 | 1.2 | 3.0 | 2.6 |
| 4.8 | 1.4 | 3.5 | 2.3 | ||
| 77–82 | 5 | 0.9 | 0.4 | 1.2 | 2.5 |
| 0.9 | 0.6 | 1.2 | 2.3 | ||
| 83–105 | 22 | 5.3 | 5.0 | 5.0 | 6.7 |
| 5.2 | 5.8 | 5.3 | 5.7 | ||
| 106–113 | 6 | 1.7 | 0.8 | 1.2 | 2.3 |
| 1.5 | 0.9 | 0.8 | 2.8 | ||
| 114–143 | 29 | 11.7 | 6.0 | 2.9 | 8.4 |
| 11.7 | 5.8 | 4.1 | 7.4 | ||
| 160–189 | 22 | 21.1 | 0.3 | 0.4 | 0.2 |
| 21.8 | 0.2 | 0 | 0 | ||
| 190–221 | 27 | 24.6 | 0.2 | 0.5 | 1.7 |
| 25.9 | 1.1 | 0 | 0 |