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Preferential interaction coefficients (Γμ3) for native BSA-destabilizing solute interactions as a function of bulk solute molality (m3bulk) calculated as described by Courtenay et al. (2000a). Panels A–C show all data for urea (at 2.70, 3.16, and 3.61 mM BSA), GuHCl (at 2.90 and 3.16 mM BSA), and GuHSCN (at 2.56, 3.16, and 3.75 mM BSA). Error bars represent the propagated experimental uncertainty. No dependence of Γμ3 on BSA concentration was observed.
