Table 1.
Comparison of polyamine content of wild-type cells and of polyamine auxotrophs grown in 10−8 M spermidine (nmol/mg protein)
| Strain | Genotype* | Doubling time, h | Putrescine | Spermidine | Spermine |
|---|---|---|---|---|---|
| Y534 | Wild type (BY4741) | 1.75 | 1 | 24 | 2.3 |
| Y535† | KANMX::spe1Δ (Y5034) | 2.1 | 0 | 0‡ | 0.03‡ |
| Y536† | KANMX::spe2Δ (Y1743) | 2.2 | 36 | 0.1‡ | 0‡ |
| Y537† | KANMX::spe3Δ (Y5488) | 2.2 | 55 | 0.1‡ | 0‡ |
One milliliter of cell culture at 1.0 OD600 contained ≈ mg of wet weight cells (≈200 μg of protein).
*With reference to polyamine biosynthetic genes. Saccharomyces Genome Deletion Project, Stanford University, Stanford, CA; Identification number (Open Biosystem) is shown in parentheses.
†The medium contained 10−8 M spermidine.
‡The limit of detection is 0.03 nmol per mg of protein, and the limit of accuracy for the assay method is 0.05 nmol spermidine or spermine per mg of protein.