Figure 2.
The interaction of daf-16 with clk-1 and eat-2. (a) The percentage of worms alive on a given day after eggs being laid (day 0) for three pooled experiments at 18°C: N2(□), daf-16(m26)(■), clk-1(e2519) (○), and daf-16(m26); clk-1(e2519) (•). Mean life span ± SEM, with sample size in parentheses, are 20.8 ± 0.4 (145), 19.0 ± 0.6 (145), 25.0 ± 0.5 (141) and 23.5 ± 0.4 (149), respectively. (b) The percentage of worms alive on a given day after eggs being laid (day 0) for two pooled experiments at 20°C: N2 (□), daf-16(m26) (■), eat-2(ad465) (○), and daf-16(m26); eat-2(ad465) (•). Mean life spans are 19.7 ± 0.5 (100), 17.4 ± 0.3 (100), 26.3 ± 0.6 (100), and 23.6 ± 0.6 (57), respectively. In one of the two trials pooled for this figure, and in Fig. 3, the last few N2 worms lived much longer than normal. In this trial, the maximum life span of eat-2(ad465) and N2 were comparable. However, in the six other trials eat-2(ad465) had a maximum life span that was clearly greater than that of N2. All other eat mutations that lengthen mean life span also clearly lengthen maximum life span.