Figure 3.
Steady-state fluorescence properties of 546- and 625-MDE. (A) Emission spectra of 546- and 625-MDE compared with l-tryptophan (excitation wavelength of 295 nm). Emission spectra were normalized for differences in concentration. The quantum yields (Φ) and emission peak maxima (λMAX) are indicated in Table 1. (B) Acrylamide quenching of 546- and 625-MDE. Relative fluorescence intensity changes from quenching (F0/F) are plotted as a function of acrylamide concentration (average of three trials ± SE). The data was fit to the Stern-Volmer relationship as described in Materials and Methods. The calculated dynamic (KSV), static (V), and bimolecular (Kq) quenching constants are shown in Table 1.